Ramadhan Sumarmin


The research to describe the ewe ovarian status post transplanted intrauterine in local pseudo pregnant rabbit had been conducted by using a descriptive method in which the 4 pieces to 4 spaces of ewe ovarian were transplanted to each of the rabbit’s cornua uterine. The description of completed ovarian post the transplanted scored with 4 (90-100% completed), 3 (70-90% completed), 2 (50-70% completed) , and 1 (<50% completed). In the research, the COCs (Cumulus Oophorus Complexes) were collected by slicing method, and then the date of the CoCs was analyzed by ANOVA and DNMRT. The result showed that all ewe ovarian in local pseudo pregnant rabbits post-transplanted were still alive until the fifth or the seventh day of transplantation. Based on the observation and statistic test, in days 5 and 7, it was found a similar status (p<0.05) of post-transplanted ewe ovarian in local and control. The percentages of NZW5 (58.82) COCs decreased significantly (p<0.05) compared to the control (50.36), NZW7 (47.52), local5 (50.42) and local7 (44.78) respectively. It can be concluded that intrauterine transplantation of ewe ovarian to the local and NZW pseudo pregnant rabbits can preserve the ovaries.

Key words: ovaries, transplantation, local rabbit, COCs


ED. 1986. The Rabbit dalam Current Theraphy in Theriogenology 2. Morrow DA. Editor. WB Sounders Co. Philadelphia.

Cushman RA, Wahl CM, and Fortune JE. 2002. Bovine ovarian cortical pieces grafted to chick embryonic membranes : a model for studies on the activation of primordial follicles. Human Reprod. 17(1), 48 – 54.

Dissen GA, Lara HE, Fahrenbach WH, and Costa MA, Ojeda SR. 1994. Immature rat ovaries becomes revascularized rapidly after autotransplantation and show a gonadotrophine-dependent increase in angiogenic factor gene expression. Endocrinology. 134(3), 1146 -1153.

Forcada F, and Lopez M. 2000. Repeated surgical embryo recovery and embryo production in rabbit. Anim Reprod Scie. 64,121 – 126.

Gilbert F. Scott. 1994. Developmental Biology. 4th edition. Sinauer Association, Inc. Sunderland, Massachusetts. P. 803-805.

Gosden RG, Boulton MI, Grant K, and Webb R. 1994a. Follicular development from ovarian xenografts in SCID mice. Reprod Fertil. 101, 619 – 623.

Gosden RG, Baird DT, Wade JC, and Webb R. 1994b. Restoration of fertility to oophorectomized sheep by ovaries autografts stored at -196 degrees C. Human Reprod. 9(4), 597 – 603.

Gunasena KT, Lakey JR, Vilines PM, Critser ES, and Critser JK. 1997. Allogeneic and xenogeneic transplantation of cryopreserved ovarian tissue to athymic mice. Biol Reprod. 57(2), 226 – 231.

Gunasena KT, Lakey JR, Vilines PM, Bush M, Raath C, Critser ES, Mc Gann LE, and Critser JK. 1998. Antral follicles develot inxenografted cryopreserved African elephant (Loxodonta africana) ovarian tissue. Anim Reprod Sci. 53, 265 – 175.

Hafez ESE. 1970. Reproduction and Breeding techniques for Laboratory Animal. Lea and Febiger. Philadelphia.

Jaswandi, Boediono A, Setiadi MA.2001. In vitro maturation and fertilization of ovine oocytes in system wiyhw absence of 5% CO2. Reprotech. 1:19-22.

Kagabu S, and Umezu M. 2001. Effect of blood transfusion on the survival rate of cryopreserved mouse ovaries transplanted into rat uterine cavity. Mamm Ova Res. 18, 58 – 61.

Kim SS, Radford J, Harris M, Varley J, Rutherford A, Lieberman B, Shalet S, and Gosden R. 2001. Ovarian tissue harvested from lymphoma patients to preserve fertility may be saving for autotransplantation. Biol Reprod. 16(10), 2056 – 2060.

Liu J, Van der EJ, Van den BR, Dumortier F, and Dhont M. 2000. Maturation of mouse primordial follicles by combination of grafting and in vitro culture. Biol Reprod. 62, 1218 – 1223.

Parkening TA, Collins TJ, and Elder FFB. 1985. Orthotopic ovarian transplantations in young and aged C57BL/6J mice. Biol Reprod. 32, 989 – 997.

Snow M, Cox SL, Jenkin G, Trounson A, and Shaw J. 2002. Generation of live young from xenografted mouse ovaries. Science. 297, 2227.

Steel RGD and Torrie JH. 1993. Prinsip Dan Prosedur Statistik. Penterjemah Soemantri. Gramedia. Jakarta.

Techakumphu M, Wintenberger-Torres S, and Sevellec C. 1987. Survival of rabbit embryos after synchronous or asynchronous transfer. Anim Reprod Scie. 12, 297 – 304.



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Sainstek: Jurnal Sains dan Teknologi
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