Analysis of the Quality of Isolated DNA in the Making of Guinea Pig DNA Test Standards

Alfi Sophian; Sri Utaminingsih; Sofia Dyah Utami

doi:10.31958/js.v15i2.7732

Analysis of the Quality of Isolated DNA in the Making of Guinea Pig DNA Test Standards

Alfi Sophian, Sri Utaminingsih, Sofia Dyah Utami

Abstract

Analysis of the quality of DNA isolated from the manufacture of species DNA standards is one way to determine the quality of independently made species-specific DNA used in testing species DNA detection. This research was conducted to assess the quality of DNA isolated from the production of guinea pig DNA test standards. The benefit of this research is to provide an alternative reference for manufacturing species DNA standards. Moreover, this research is expected to enrich the methods of testing the quality of DNA isolated from different existing methods, using various test matrices. The DNA isolation method was carried out using the Dneasy Mericon Food kit. The quality of the isolated DNA was analyzed using a nanophotometer, measuring the parameters of concentration and purity values at the A260/A280 ratio. The results of DNA isolation indicated a concentration value of the isolated DNA ranging from 230.2 ng/µL to 238.5 ng/µL, with an average concentration value of 233.7 ng/µL. The purity value, read at the A260/A280 ratio, falls within the range of 2.04 – 2.11. Based on the study's results, it was found that the isolated DNA exhibited good DNA quality, making the DNA test standard suitable for use as a benchmark in species DNA testing.

Keywords

A260/A280; Analysis; DNA; Species; Test

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DOI: http://dx.doi.org/10.31958/js.v15i2.7732