AKTIVITAS AMILASE BAKTERI YANG DIISOLASI DARI SUMBER AIR PANAS CISEENG BOGOR

Erismar Amri, Nunuk Widhyastuti, I Made Artika

Abstract


This research was aimed at studying the activities of amylase produced by isolate bacteria which were isolated from hot water resource, Ciseeng Bogor. Four among the nine isolate bacteria grown on NA media showed the activities of amylase. The character of Amylase was analyzed in 2 isolates, A1 and A3. Isolate A1 was optimally active at pH 3, 55 C, meanwhile isolate A3 was optimally active at pH 4, 50 C. The amylase resulted from either isolate A1 or isolate A3 was acid amylase. Both of the isolates were relatively stable at pH acid, whereas the amylase resulted from A3 was not stable on pemanasan in 30 minutes, and the amylase from A1 increased after being incubated at more than 60 C in 30 minutes. In conclusion, isolate A1 identified as Bacillus Coagulans indicated the optimal activities when it was grown at pH 6 and at the 14th hour of incubation.

Key words: amylase, isolation, enzyme           


References


Artika IM. 1 Juli 1993. Menjadikan Mikroba Sebagai Pabrik Enzim. Kompas Kolom (1-6).

Bernfield P. 1955. Amilase α and β. Dalam Colowick, S.P. and N.D. Kaplan (eds). New York: Methode in Enzimology and Related of Biochemistry. Academic Press.

Breed RS, Murray EGD, Hitchens AP. 1948. Bergey’s Manual of determinative Bacteriology. The Williams & Wlkins Company.

Cruerger W, Cruerger A. 1984. Biotechnolgy: A text Book of Industrial Microbiology. USA: Sience tech.

Girindra A. 1990. Biokimia 1. Jakarta: Gramedia.

Harper HA, Rodwel VW, Mayer PA. 1984. Review of Physiological Chemistry. Lange Medical Publication, California USA.

Lehninger AL. 1995. Dasar-dasar Biokimia. Terjemahan Meggy Thenawijaya. Jakarta: Erlangga.

Lestari P, Darwis AA, Syamsu K, Richana N Damardjati DS. 2001. Analisis Gula Reduksi Hasil Hidrolisis Enzimatik pati Ubi Kayu oleh α-amilase Termostabil dari Bacillus stearothermophilus TII12. Jurnal Mikrobiologi Indonesia.

Nuraida FR. 2001. Isolasi dan Seleksi Bakteri Amilolitik. Laporan Kerja Praktik. Jurusan Biologi FMIPA. Bandung: Universitas Padjadjaran.

Palmer T. 1981. Understanding Enzymes. Ellis Horwood. Ltd, England.

Pelczar MJ, Chan ECS. 1988. Dasar-dasar Mikrobiologi. Terjemahan Ratna Siri, dkk. Jakarta: UI_Press.

Suhartono MT. 1989. Enzim dan Bioteknologi. Bogor: PAU. Bioteknologi.

Tauber H. 1950. Chemistry and Technology of Enzyme. New York: John wiley and Sons.




DOI: http://dx.doi.org/10.31958/js.v2i1.6

Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Erismar Amri, Nunuk Widhyastuti, I Made Artika

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Indexed by:

       

 

__________________________________________________________________________

Sainstek: Jurnal Sains dan Teknologi
ISSN 2085-8019  (print) | 2580-278x  (online)
Published by Institut Agama Islam Negeri Batusangkar

Email: sainstek@iainbatusangkar.ac.id


View Sainstek Stats

 

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.