Yuni Ahda, Lel Fitri


The research objective is to locate and determine the morphological and biochemical characteristics of the bacteria that could potentially degrade the used lubricant oil in the workshops in Padang. The research was conducted March to October 2016. The bacteria obtained from the workshop is cultured on selective media MSM and transferred to LB medium to obtain pure isolates. Morphological and biochemical characterization indicate three types of bacteria that live in workshop’s soil contaminated used lubricant oil, namely Bacillus sp1, sp2 and Alcaligenes Bacillus sp


biochemistry characterization, Bacillus, Alcaligenes


Bumpus JA. 1989. Biodegradation of polycyclic aromatic hydrocarbon by Phanerochaete chrysosporium. Applied and Environmental Microbiology; 55(1):154-158

Cerniglia CE and Sutherland JB. 2001. Bioremediation of polycyclic aromatic hydrocarbon by ligninolytic and non-ligninolytic fungi. In Fungi in Bioremediation. Gadd GM. Ed. 136-187. Cambridge University Press, Cambridge, UK.

Clemente AR, Anazawa TA, Durrant LR. 2001. Biodegradation of polycyclic aromatic hydrocarbon by soil fungi. Brazilian Journal of Microbiology; 32(4):255-261.

Gofar N. 2012. Aplikasi Isolate Bakteri Hidrokarbonoklastik Asal Rizosfer Mangrove pada Tanah Tercemar Minyak Bumi. Jurnal Lahan Suboptimal; 1(2):123-129.

Husaini A, Roslan HA, Hii KSY, Ang CH. 2008. Biodegradation of aliphatic hydrocarbon by indigenous fungi isolated from used motor oil contaminated sites. World Journal of Microbiology and Biotechnology; 24(12):2789-2797.

Llyod AC. and Cackette TA. 2001. Diesel engines: environmental impact and control. Journal of the Air and Waste Management Association; 51(6):809-847.

Mishra S., Jyot J., Kuhad RC., Lal B. 2001. Evaluation of inoculums addition to stimulate in situ bioremediation of oily-sludge-contaminated soil. Applied and Environmental Microbiology; 67(4):1675-1681.

Nusyirwani dan Amolle KC. 2007. Isolasi dan Karakterisasi Bakteri Hidrokarbonoklastik dari Perairan Dumai dengan Sekuens 16S rDNA. Ilmu Kelautan; 12(1):12-17.

Propst TL, Lochmiller RL, Qualls CW, Mcbee K. 1999. In situ (mesocosm) assessment of immunotoxicity risks to small mammals inhabiting petrochemical waste sites. Chemosphere; 38(5):1049-1067.

Schallmey, Marcus, Singh, Ajay, Ward, Owen P. 2004. Developments in The Use of Bacillus species for Industrial Production. Canadian Journal of Microbiology, 50(1):1-17.

Shida O, Takagi H, Kadowaki K, Komagata K. 1996. Proposal for Two New Genera, Brevibacillus gen.nov and Anouriniba bacillus gen.nov. International Journal of Systematic Bacteriology, 46(4):939-946.

Van Hamme JD, Singh A, Ward OP. 2003. Recent advances in petroleum microbiology. Microbiology and Molecular Biology Reviews; 67(4):503-549.

Zam SI. 2006. Bioremediasi Limbah Pengilangan Minyak Bumi Pertamina UP II Sungai Pakning dengan Menggunakan Bakteri Indigen. Program Studi Bioteknologi, ITB. Bandung. Ganesha Digital Library.

DOI: http://dx.doi.org/10.31958/js.v8i2.470


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