Isolasi dan Karakterisasi Enzim Phytase Mikroflora Endofitik Tanaman Kedelai (Glycine max (L.) Merril)

RINA DELFITA

Abstract


Two endophytic fungal phytases is isolated from leaf, stem and root fragments collected from soybean and identified as Rhizoctonia sp. and Fusarium verticillioides. The phytase production was induced by phytate in medium used. The crude preparations were used in subsequent characterisation studies, pH and temperature optima and compared to other phytases tested, and is thus a promosing candidate for animal feed applications. This particular phytase retains activity over a wide range pH value characteristic of the digestive tract and could conceivably be more suited to increasingly hinger feed processing temperatures being today, than corresponding phytase from Aspergillus niger.  

 

Key words: Endophytic fungal phytase, soybean, Rhizoctonia sp., Fusarium verticillioides, characterisation

References


Barnett HL and Hunter BB. 1972. Ilustrated Genera of Imperfect Fungi. Third Edition. Burgess Publishing Company. USA.

Cao LW, Wang C, Yang Y, Yang J, Diana A, Yakupitiyage Z, Luo and Lie D. 2007. Application of Microbial Phytase in Fish Feed. J. Enzyme and Microbial Technology. 40: 497-507.

Cheryan M. 1980. Phytic Acid Interactions in Food Systems. Food Sci. Nutr. 13: 297-335.

Champell NA, Reece JB and Mitchell LG. 2000. Biology. Fifth Edition. Addison Wesley Longman. California.

Choi YM, Suh HJ and Kim JM. 2001. Purification and Properties of Extra-sellular Phytase from Bacillus sp.. J. Protein Chem. 20 (4): 287-292.

Fujita J, Yamane YI, Fukada H, Kizaki Y, Wakabayashi S, Shigata S, Suzuki O and Kono, 2003. Production and Properties of Phytase and Acid Phosphatase from a Sake Koji Mold, Aspergillus oryzae. Jour. Bioscience and Bioenginering. 95(4): 384-353.

Graf E, Empson K and Eaton JW. 1987. Phytic Acid. A Natural Antioxidant. J. Biol. Chem. 262: 11647-11651.

Greiner R, Konietzny U and Jany KD.1993. Purification and Characterization of Two Phytases From Escherichia coli. Arch. Biochem. Biophys. 303: 107-113.

Greiner R, Haller E, Konietzny U and Jany KD. 1997. Purification and Characteri-zation of A Phytase From Klebsiella Terrigena. Arch. Biochem. Biophys. 341: 201-206.

Greiner R and Konietzny U. 2006. Phytase for Food Aplication. Food Technol. Biotechnol. 44 (2): 125-140.

Howson SJ and Davis RJ. 1983. Production of Phytate-Hydrolysing Enzyme by Fungi. J. Enzyme Microbiol. Technol. 5: 377-382.

Irving GCJ and Cosgrove DJ. 1971. Inositol Phosphate Phosphatase of Microbial Origin. Observations on The Nature of The Active Center of A Bacterial (Pseudomonas sp.) Phytase. Austral. J. Biol, Sci. 24: 1559-1564.

IUPAC-IUB (Commission on Biochemical Nomenclature). 1977. Nomenclature of phosphorus containing compounds of biochemical importance. Eur. J. Biochem. 79: 1-9.

Kerovuo J. 2000. A Novel Phytase from Bacillus: Characterization and Producti-on of Enzyme. Dissertation of Doctoral at Faculty of Science of the University of Helsinki. Firlandia.

Kim YO, Kim HK, Bae KS, Yu JH and Oh TK. 1998. Purification and Properties of a Thermostable Phytase From Bacillus sp. Ds11. Jour. Enzyme Microbiol. Technol. 22: 2-7.

Kim HW, Kim YO, Lee JH, Kim KK, Kim YJ. 2003. Isolation and Characteri-zation of a Phytase with Improved Properties from Citrobacter braakii. Biotechnol. Lett. 25: 1231-1234.

Kim TW and Lei XG. 2005. An Improved Method for a Rapid Determination of Phytase activity in animal feed. J. Anim. Sci. 83: 1062-1067.

Konietzny U and Greiner R. 2002. Molecular and Catalityc Properties of Phytat Degrading Enzyme (Phytase). Int. J. Sci. Technol. 37: 791-812.

Konietzny U and Greiner R. 2004. Bacterial Phytase: Potential Aplication, In Vivo Function and Regulationof Its Syn-thesis. Braziliat. Jour Microbiol. 35: 11-18.

Laboure AM, Gagnon J and Lescure AM. 1983. Purification and Characterization of A Phytase (Myo-Inositol Hexakis-phosphate Phosphohydrolase) Accumu-lated in Maize (Zea mays) Seedlings During Germination. Biochem. J. 295: 413-419.

Lan GQ, Abdullah N, Jalaludin S and Ho YW. 2002. Culture Conditions In-fluencing Phytase Production of Mitsuokella jalaludinii, a New Bacterial Species from the Rumen of Cattle. J. Appl. Microbiol. 93: 668-674.

Lan GQ, Abdullah N, Jalaludin S and Ho YW. 2004. Efficacy of Suplementation of Phytase-Producing Bacterial Culture on the Performance and Nutrient Use of Broiler Chickens Feed Corn-Soybean Meal Diets. Poultry Science. 81: 1522-1532.

Liu Z, Wang H, Xiu-E Wang, Xu H, Gao D, Zhang G, Chen P and Liu D. 2007. Effect of Wheat Perling on Flour Phytase Activity, Phytic Acid, Iron and Zinc Content. Food Sci and Technology. Swiss.

Lolas GM and Markakis P. 1977. Phytic Acid and Other Phosphorus Compound Soybeans (Phaseolus vulgaris). J. Agric. Food. Chem. 23: 13.

Marlida Y. 2001. Isolation and Purification of Raw Starch Degrading Enzyme from Acremonium endophytic Fungus and Its Aplication for Glucosa Production. Dissertation of Doctoral at University Putra Malaysia. Malaysia.

Marlida Y. 2003. Penampilan Produksi Ternak Domba Menggunakan Pakan Berserat Tinggi yang Difermentasi dan Disuplementasi dengan Enzim Selulase yang Dihasilakan oleh Kapang Endo-fitik. Laporan HB. X/II. Padang.

Martin JA, Murphy RA and Power RFF. 2005. Purrification and Physico-Chemical Characterisation of Gene-tically Modifed Phytases Expressed in Aspergillus awamori. Bioresource Technology 97. Irlandia.

Pallauf J and Rimbach RFF. 1996. Nutritional Significance of Phytic Acid and Phy-tase. Arch. Anim. Nutr. 50: 301-319.

Pimentel IC, Glienke-blanco C, Gabardo J, Stuard RM and Azevedo JL. 2006. Identification and Colonization of Endophytic Fungi from Soybean (Glycine max (L.) Merril) under Different Environmental Conditions. Jour Brazilian Archieves of Biology and Technilogy. 45 (5): 705-711.

Powar VK. and Jagannathan V. 1982. Purfication and Properties of Phytate-Specific Phosphatase from Bacillus subtilis. J. Bacteriol. 151: 1102-1108.

Quan C, Zhang L, Wang Y and Ohta Y. 2001. Production of Phytase in Low Phosphate Medium by a Novel Yeast Candida krusei. Journal of Bioscience and Bioenginering. 9 (2): 154-160.

Radji M. 2005. Peranan Bioteknologi dan Mikroba Endofit dalam Pengembangan Obat Herbal. Majalah Ilmu Kefarmasi-an. Desember 2005. 2 (3): 112- 126.

Reddy NR, Pierson MD, Sathe SK and Salunkhe DK. 1989. Phytates in Cereals and Legumes. Crc Press, Inc., Boca Raton, Fla.

Rhodes A and Fletcher DL. 1966. Principles of Industrial Microbiology. Pergamon Press. New York.

Samson RA and van Reenen ES-Hoekstra. 1988. Intoduction to Food-Borne Fungi. Centraalbureau voor Schimmel-cultures. Wageningen.

Sandberg AS and Svanberg U. 1991. Phytate Hydrolysis by Phytase in Cereals: Effect on in vitro Estimation of Iron Availability. Jour. of Food Science. 56 (5): 1330-1333.

Scott JJ. 1991. Alkaline Phytase Activity in Nonionic Detergent Extracts of Legume Seeds. Plant Physiol. 95, 1298-1301.

Selle PH, Ravindran V, Cadwell RA and Bryden WL. 2000. Phytate and Phytase: Concequences for Protein Utilisation. Nutrition Reviews: 255-278.

Shieh TR and Ware JH. 1968. Survey of Microorganisms for The Production of Extracellular Phytase. Appl. Microbiol. 16: 1348-1351.

Shah V and Parekh LJ. 1990. Phytase From Klebsiella sp. No. Pg-2: Purification and Properties. Indian J. Biochem. Biophys. 27: 98-102.

Shimizu, M. 1992. Purfication and Characteri-zation of Phytase From Bacillus subtilis (Natto) N-77. Biosci. Biotechnol. Biochem. 56: 1266-1269

Shaefer A and Koppe WM. 1995. Effect of Microbial Phytase on Utilization on Native Phosporus by Carp in a Diet Based on Soybean Meal. Journ of Water Science Technol. 31 (1): 159-155.

Singh M and Krikorian AD. 1982. Inhibiton of Trypsin Activity in vivo by Phytate. J. Agric. Food Chem. 30: 799-805.

Simell M, Turunen M, Piironen J and Vaara T. 1989. Feed and Food Applications of Phytase. Lecture At 3rd Meet. Industrial Applications Of Enzymes, Barcelona, Spain.

Simoes NC and Guggenbuhl P. 1998. Comparative Effect of Aspergillus fumigatus and Aspergillus niger on Phosphorus and Calcium Digestibilities and Phosphorus Faecal Excretion in Growing Pig. J. Anim. Feed Sci. 71: 177-180.

Trudy, McKee. 1999. Biochemistry: An Intoduction. Secend Edition. McGraw-Hill. Amerika.

Stefan H, Anja K, Edzard S, Bijoerg S, Marcus L, Oskar Z. 2005. Biotechno-logical Production and Application of Phytase. App. Microbiol Biotech. 68 (5): 125-140.

Volfova O, Dvorakova J, Hanzlikova A and Jandera A. 1994. Phytase from Aspergillus niger. Folia Microbiol. 39: 481-484.

Vohra A and Satyanarayana T. 2002. Purification and Characterization of Thermostabil and Acid Stable Phytase from Pichia anomala. Jour. Micro and Biotech. 18: 687-691.

Wards OP. 1983. Proteinase. In: Forgarty WM (ed). Microbial Enzyme and Biotechnology. London.

Walz OP and Pallauf J. 2002. Microbial Phytase Combined with Amino Acid Suplementation Reduced P and N Excretion og Growing and Finishing Pig without Loss Performance. Int. J. Food Sci. Technol. 37: 835-848.

Widowati S, Rosmimik S, Andriani D dan Damardjati DS. 1998. Optimalisasi Produksi Phytase dari Bacillus coagulans pada Skala Laboratorium. Makalah Disampaikan pada Seminar Nasional Bioteknologi di Malang.

Wodzinski RJ and Ullah AHJ. 1996. Phytase. Adv. Appl. Microbiol. 42: 263-303.

Wyss ML, Pasamontes, Friedlein A, Remy R, Tessier M, Kronenberger A, Middendorf A, Lehmann M, Shnoebelen L, Rothtlisberger U, Kusznir E, Wahl G, Muller F, Lahm HW, Vogel K and Van Loon. 1999. Biophysical Characterization of Fungal Phytases (Myo-Inositol Hexakispho-sphate Phosphohydrolases): Molecular Size, Glycosylation Pattern, and Engineering of Proteolytic Resistance. Appl. Environ. Microbiol. 65: 359-366.

Yang WJY, Matsuda S, Sano H, Masutani and Nakagawa. 1991. Purification and Characterization of Phytase From Rat Intestinal Mucosa. Biochim. Biophys. Acta. 1075: 75-82.

Yanke LJ, Bae HD, Selinger LB, Cheng KJ. 1998. Phytase Activity of Anaerobic Ruminal Bacteria. Microbiol. 144. 1565-1573.

Yoon SJ, Choi YJ, Min HK, Cho KK, Kim JW, Lee SC and Jung YH. 1996. Isolation and Identification of Phytase-Producing Bacterium, Enterobacter sp. 4, and Enzymatic Properties of Phytase Enzyme. Enzyme Microbiol. Technol. 18: 449-454.




DOI: http://dx.doi.org/10.31958/js.v3i1.38

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