Antibacterial Effectiveness of Methanol Extract Combination Formula 3:2:1 of Tambora Leaf (Ageratum conyzoides), Sembalit Angin Leaf (Mussaenda frondosa L), Turmeric Rhizome (Curcuma longa L) on the growth of Staphylococcos aureus

Ardiansyah Ardiansyah, Noor Hujjatusnaini, Astuti Muh. Amin, Lilin Ika Nur Indahsari

Abstract


The research was a quantitative descriptive study using laboratory experimental methods. We conducted this research to analyze the combination formula of Tambora leaves, Sembalit Angin leaves, and turmeric rhizomes using 3: 2: 1 ratio and determine its effectiveness in-inhibiting the-growth of Staphylococcus aureus bacteria in vitro. The width of the inhibition zone measured the growth of Staphylococcus aureus from the outer side of the paper disc, which has been immersed in methanol extract of Tambora leaves, Sembalit Angin leaves, and turmeric rhizomes in nutrient agar medium. Calipers did measurement, which were made during the incubation period of 24 hours, 48 hours, and 72 hours. The data obtained from the effect of a combination formula of 3:2:1 Tambora leaves, Sembalit Angin leaves, and turmeric rhizomes on the growth of Staphylococcus aureus were analyzed by Anava statistical test, and  Duncan 1% test. The results found that the combination formula of 3:2:1 bio herbal methanol extract of Tambora leaves, Sembalit Angin leaves, and Turmeric rhizomes had a significant effect on the growth of Staphylococcus aureus at 1% significance, proven by the sig value. 0.00 <0.01, so the 3: 2: we can recommend 1 combination formula as the most effective combination extract formulation in inhibiting the growth of Staphylococcus aureus.

Keywords


Combination Formula; Ageratum conyzoides; Mussaenda frondosa; Curcuma longa; Staphylococcus aureus.

Full Text:

PDF

References


Ardhany, S. D., & Novaryatiin, S. (2019). Pendampingan Pembuatan Krim Bawang Dayak Khas Kalteng Sebagai Anti Acne di SMK Farmasi Muhammadiyah Palangka Raya. PengabdianMu: Jurnal Ilmiah Pengabdian Kepada Masyarakat, 4(2), 111–116.

Garvita, R. V. (2015). Pemanfaatan Tumbuhan Obat Secara Tradisional untuk Memperlancar Persalinan oleh Suku Dayak Meratus di Kalimantan Selatan. Warta Kebun Raya, 13(2), 51–58.

Halimatussa’diah, F., Fitriani;, V. Y., & Rijai, L. (2014). Aktifitas Antioksidan Kombinasi Daun Cempedar (Artocarpus champedan) dan Daun Bandotan (Ageratum conyzoides). 2(5), 248–251.

Hayati, D. D., Isa, M., & Harris, A. (2020). 12. Antibacterial Activity of Ethanol Extract of Siamih Leaf (Ageratum conyzoides) on Staphylococcus aureus bacteria. Jurnal Medika Veterinaria, 14(1), 88–98.

Hidayati, & Harjono. (2017). Uji Aktivitas Antibakteri Krim Ekstrak Daun Babadotan (Ageratum conyzoides. L) dalam Pelarut Etanol. Jurnal MIPA, 40(1), 33–38.

Iqlima, D., Ardiningsih, P., & Wibowo, M. A. (2017). Aktivitas Antibakteri Isolat Bakteri Endofit B2D dari Batang Tanaman Yakon (Smallanthus sonchifolius (Poepp. & Endl.) H. Rob.) Terhadap Bakteri Staphylococcus aureus dan Salmonella thypimurium. Jkk, 7(1), 36-43.

Jalil, M. (2019). Keanekaragaman dan Asas Manfaat Keluarga Zingiberaceae di Dusun Jambean Kabupaten Grobogan. Life Science, 8(1), 75–85.

Jumiati, J., Ratnasari, D., & Sudianto, A. (2019). Pengaruh Penggunaan Ekstrak Kunyit (Curcuma domestica) Terhadap Mutu Kerupuk Cumi (Loligo sp.). Jurnal Ilmiah Perikanan Dan Kelautan, 11(1), 55.

Kholifah, N. (2018). Uji Aktivitas Antibakteri Kombinasi Ekstrak Etanol Rumput Bambu (Lophatherum gracile Brongn) dan Rimpang Kunyit Putih (Curcuma zedoaria (Berg.) Roscoe) Terhadap Staphylococcus aureus dan Escherichia coli. 1-26.

Mas’udah, E. K. (2017). Pengaruh Ekstrak Daun Turi Merah Terhadap Penurunan Kadar Tnf-A, ll-1β dan Jumlah Koloni Bakteri Pada Ovarium Mus musculus Nifas Yang Diinokulasi Staphylococcus aureus. 1-95.

Novaryatiin, N. Q. R. H. S., & Universitas. (2011). Peningkatan Pengetahuan dan Keterampilan Ibu Rumah Tangga dalam Pengolahan Tanaman Obat Keluarga ( Toga) Sebagai Ramuan Obat Tradisional Increased Knowledge and Skills of Housewives in Processing Family Medicinal Plants ( Toga ) As Traditional Medicines.

Safani, E. E., Kunharjito, W. A. C., Lestari, A., & Purnama, E. R. (2019). Potensi Ekstrak Daun Bandotan (Ageratum conyzoides L.) Sebagai Spray Untuk Pemulihan Luka Mencit Diabetik yang Terinfeksi Staphylococcus aureus. Biotropic : The Journal of Tropical Biology, 3(1), 68-78.

Saputra, H., Dermawan, Y., & Wati, S. L. (2019). Sabun Cair Berbahan Dasar Olein Kelapa Sawit dengan Penambahan Ektrak Bandotan (Ageratum conyzoides L. ). Jurnal Citra Widya Edukasi, 11(3), 223-230.

Sudewi, S., & Lolo, W. A. (2016). Kombinasi Ekstrak Buah Mengkudu (Morinda citrifolia L.) dan Daun Sirsak (Annona muricata L.) dalam Menghambat Bakteri Escherichia coli dan Staphylococcus aureus. Kartika Jurnal Ilmiah Farmasi, 4(2), 36-42.

Sugara, T. H., Irawadi, T. T., Suprapto, I. H., & Hanafi, M. (2016). Uji Aktivitas Antibakteri Fraksi Etil Asetat Daun Tanaman Bandotan ( Ageratum conyzoides L ) Anti Bacteria Activity of Ethyl Acetate Fraction Bandotan leaf ( Agerantum conyzoides L ). Jurnal Ilmiah Ibnu Sina, 1(1), 88-96.




DOI: http://dx.doi.org/10.31958/js.v13i1.3513

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Noor Hujjatusnaini

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Indexed by:

       

 

__________________________________________________________________________

Sainstek: Jurnal Sains dan Teknologi
ISSN 2085-8019  (print) | 2580-278x  (online)
Published by Institut Agama Islam Negeri Batusangkar

Email: sainstek@iainbatusangkar.ac.id


View Sainstek Stats

 

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.